This course consists of online self-paced modules and activities.

Duration: 12 hours

Items needed: Reverse phase test mix and C18 column (included with instrument purchase, P/N: 220-91394-02), LC Grade Water, and LC Grade Methanol

This course covers the skills required by an analyst to successfully analyze unknown samples using a Shimadzu LC-20 or LC-30 system with LabSolutions software. Students will learn to operate the HPLC hardware, create methods, acquire and process data, and calibrate methods for quantitative analysis. Troubleshooting is also discussed.

Learning outcomes are the competencies the learner will acquire during this course. At the end of this course the learner will be able to:

1) List and explain the functionality of each part of the HPLC system

2) Demonstrate proper instrument start-up, purging and equilibration

3) Create effective and efficient isocratic and gradient methods

4) Perform data acquisition with LabSolutions software

5) Process data files in LabSolutions and apply correct integration

6) Create a calibration curve and quantitate an unknown sample

7) Describe how hardware and method parameters affect a chromatogram

8) Demonstrate the ability to identify and correct common HPLC problems

This course is online only.